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Runtogen

Overexpression Cell Line Service

Overexpression Cell Line Service

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Overexpression Cell Line Service

 

Runtogen specializes in engineering cell lines for stable overexpression of your gene of interest through Lentivirus transduction or PiggyBac transposition. Leveraging years of expertise in gene editing, we have developed a comprehensive, centralized cell repository designed to streamline your research. Our proprietary lentiviral platform ensures efficient, reliable overexpression, providing a valuable resource to accelerate your scientific investigations. Runtogen’s Overexpression Cell Line Service provides a powerful solution for studying gene function, protein interactions, and cellular pathways by introducing high levels of target gene expression. By utilizing cutting-edge gene-editing technologies, we create stable and transient overexpression models that enable you to analyze the impact of increased gene expression on cellular behavior.

 

Types of Cells: 

 

  • * Tumor cell lines

  • * Stem cells

  • * IPS cells

  • * Non-stem cell lines

Work Flow and Validation: 

Host Cell Line Evaluation

1-2 weeks

  •  Host cell line will be tested for bacteria, fungi, mycoplasma and other contaminants.
  • Single clone formation efficiency will be tested to determine cell proliferation.

Vector Construction

1-2 Weeks

 

Gene subcloning in a high performing expression vector.

        Lentivirus Packaging

Host cell transfection and generation of stable pools

2-3 Weeks

 

  • Stable transfection
  • Stable Pool selection 

Postive clones screening&Cell expansion

2-3 Weeks

 

  • Characterization of clones
  • QC report

Why Choose Our Overexpression Cell Line Service?

 

  • * Custom Solutions: Every project is unique, and we work closely with you to design overexpression systems that align with your specific research objectives.

  • * High Expression Efficiency: We ensure robust, high-level gene expression with optimized vectors and advanced delivery methods, providing reliable, reproducible results.

  • * Flexibility & Reliability: Whether you need transient overexpression for short-term studies or stable overexpression for long-term research, we provide solutions tailored to your timeline and experimental needs.

  • * Comprehensive Support: From vector design to post-expression analysis, we offer full technical support throughout your project to ensure successful outcomes.

 
 

Case Study: Human TREM2 and Human DAP12 genes were overexpressed in HEK293 cell line

LV-EF1a>hTREM2/EGFP-PGK>Puro

 

Vector Map


LV-EF1a>hDAP12/MyC-DDK-PGK>Puro

 Vector Map


 

Case Study 2: Custom Human HLA-DQB2 Overexpression HeLa Cell Line

 

Vector Map:

 

 

DQB2 melt curve

β-actin melt curve

 

Gene Expression rate (%)

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