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Runtogen

Microorganisms Gene Editing Service

Microorganisms Gene Editing Service

Microorganisms Gene Editing Service

 

Microbial genome editing is a powerful way to tailor-make several important products for industries including food, medicine and industrial chemical production. we provide state-of-the-art microbial genome editing services to accelerate your research and innovation. Whether you’re engineering microbes for industrial applications, developing novel biotherapeutics, or exploring the frontiers of synthetic biology, our cutting-edge technologies and expertise ensure precise, efficient, and reliable genome modifications tailored to your needs.

 

Runtogen has developed a unique CRISPR-M platform for editing microorganisms’ genome with a wide range of microbe species. With years of experience in CRISPR based genome editing field, we could provide you the excellent services for Microbial genome editing (knockout/point mutation/knockin/overexpression) in Escherichia coli, Salmonella and Pseudomonas aeruginosa. We guarantee the delivery of positive homozygous clones for your project.

 

Microbe Species For Microbial Gene Editing Services:

 

  • Escherichia coli
  • Salmonella
  • Pseudomonas aeruginosa
  • Staphylococcus aureus

Service type: Knockout, Overexpression, Point mutation, Knockin

 

Work Flow and Validation: 

Vector Construction

* Cas9 nuclease and Red recombinase

* Target gene gRNA

* Donor template

Electroporation

 Cas9 nuclease and Red recombinase vector will be transfered into bacteria by electroporation and select positive strains

Vector transfection and generation of positive clones

 

  • gRNA vector  transfection
  • Clone selection 

Postive clones screening

 

Validate the single clones by PCR and sequencing

Why Choose RuntogenMicrobial genome editing?

Ø  Technical Support From Start to Finish

Ø  100+Successful Gene-editing services

Ø  Fast Turnaround Time

Ø  Weekly project update

Ø  Comprehensive QC: Colony PCR and sequencing

What you will receive:

  • Biweekly project updates
  • Single clone
  • Final report containing details of the vector construction and the results of all QC and validation assays.

Case Study: ku80 Knockout in Kluyveromyces lactis


 

 

 

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