Runtogen
Online Inquiry 
1-303-522-8502 
:[email protected]
Online Inquiry 1-303-522-8502 :[email protected]
Runtogen’s MagCRISPR™ KO Cell Line Service is a specialized offering designed to help researchers create cell lines with specific genes knocked out, tailored to their experimental needs. CRISPR Knockout Cell Line service is ideal for studying gene function, disease mechanisms, drug target validation, and more.
CRISPR gene editing is most direct and fastest approach to achieve the knockout of a specific gene or double genes. Runtogen’s CRISPR Gene Knockout Cell Line Service provides end-to-end support for generating custom knockout cell lines using advanced CRISPR/Cas9 gene-editing technologies. Researchers can specify the gene(s) of interest, the cell type, and the level of validation required. Our expert team utilizes CRISPR/Cas9 technology to tailor genome editing to your specific requirements. Whether you require permanent knock-out or conditional knock-out, we have you covered.
CRISPR/Cas9
Synthetic Modified sgRNA (Standard)
Frameshift, stop codon, fragment deletion; Single, Double, or Triple Knockout
Knockout Cell Line Service Working Flowchart:
We utilize advanced genome editing technologies, including CRISPR/Cas9, TALENs, and ZFNs, to achieve precise and efficient gene knockouts with high specificity.
Our knockout cell lines undergo rigorous validation at the DNA, RNA, and protein levels, ensuring reproducible and reliable experimental outcomes.
We tailor the knockout process to accommodate various cell lines and research objectives, providing flexible solutions for diverse experimental needs.
Our experienced team offers comprehensive guidance, assisting with experimental design, troubleshooting, and technical support throughout your project.
Case Study: Human GAL Knockout model in HCT116 Cell Line Service
GAL locus in human colon cancer cell line, HCT116, was knocked-out by creating indels in exon 3, as shown in the targeting strategy.
PCR Identification for positive clones
Individual clones were Sanger sequenced to select for homozygous knock-out indels of GAL at exon 3.
Sanger sequencing data. del 251 bp (E3 is completely deleted)
Cell Lines to be edited: Runtogen provides a wide collection of Tumor cell lines and othe cell lines for the customers to choose from.
The Gene Information(ID or Sequence)
It takes 1-5 months depending on the recovery of the cell lines, the proliferation rate and transfection efficiency of the cells, and validation assays requested by the customer. Speedy turnaround as fast as 4 weeks!
Exclusive innovative technique, Gene-editing efficiency improved by 10-20 times. With MagCRISPR™, Runtogen has successfully modified 8,000+ genes from more than 200 cell lines with our exclusive innovation MagCRISPR™ technology.