Knockin Cell Line Service

Targeted genome editing events fall into two general classes: knock-in and knock-out. CRISPR gene knock in cell line services are mainly divided into point mutation introduction service and tag knock in to the target gene. Point mutation cell line can be used to study the function of point mutation in situ in cells. Tag knock in cell line can locate and quantify the target protein.

Knock-in mutations typically require an exact DNA sequence to be incorporated at a precise genomic location, with little to no room for error. Our expert team utilizes a robust, automated CRISPR platform for knock-in cell lines that achieve high editing efficiencies while maintaining quality to ensure pluripotency. To enable your discoveries, Runtogen offers high quality knock-ins that generate single nucleotide variants (SNVs), tags, and <100 bp insertions, in homozygous or heterozygous states and clone or pool formats.

KI Cell Line Service Capabilities:

Type of Gene Editing

• CRISPR/Cas9

CRISPR Design

• Synthetic Modified sgRNA (Standard)

Types of Cells

• Tumor cell lines
• Stem cells
• IPS cells
• Non-stem cell lines

Available Edits

Ø  Point mutation (SNP) knock-in

Ø  Tag / Reporter knock-in:

Ø  Large knock-in