Knockdown Cell Line Service | Precision Gene Silencing Solutions

Knockdown cell lines are engineered cell lines in which the expression of a specific gene has been intentionally reduced or silenced. This is typically achieved through RNA interference (RNAi) technologies, such as siRNA (small interfering RNA) or shRNA (short hairpin RNA). Knockdown cell lines are crucial tools in functional genomics, as they enable scientists to explore the effects of reduced gene expression on cellular behavior. Unlike complete gene knockout models, where the gene is entirely deleted, knockdown cell lines provide a more nuanced approach by allowing partial gene silencing. This makes them valuable for studying genes involved in complex diseases, drug resistance, cancer, and other biological processes.

Runtogen offers a variety of Knockdown Cell Line Services tailored to meet your specific research needs. We have developed the unique MagKD™ Knockdown Cell Line Service platform for gene silencing. Utilizing advanced RNA interference (RNAi) technologies, including siRNA (small interfering RNA) and shRNA (short hairpin RNA), we provide powerful tools for gene knockdown. Additionally, we also offer custom Inducible Knockdown Cell Lines, leveraging systems like Tet-On/Tet-Off or Cre-LoxP to enable gene silencing in response to specific inducers (e.g., doxycycline). Whether you’re exploring functional genomics, conducting drug discovery, or developing disease models, Runtogen delivers stable, reliable knockdown cell lines that are tailored to meet your unique research needs.

Available Services: 

* Inducible Knockdown: Gene silencing can be controlled by adding or removing an inducer (like doxycycline) to turn the knockdown on or off using TET-ON or TET-OFF systems

* Stable Knockdown (shRNA): The gene is permanently silenced (or semi-permanently) by integrating an shRNA or other knockdown construct into the cell’s genome.

Work Flow and Validation: