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| Product Name | NFkB-Luc/Jurkat Reporter Cell Line |
| Cat.No | RG-582 |
| Size | 1*10^6 cells/vial |
| Description | NF-kB luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-kB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene. II. Introduction Host Cell: Jurkat Expressed gene: NF-κB Luciferase Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) Synonym(s): Nfkb, nf-kb reporter, nfkb report, nf-kb jurkat Freeze Medium: 90% FBS+10% DMSO Culture Medium: RPMI-1640+10%FBS+800ug/ml hygromycin Mycoplasma Testing: Negative Storage: Liquid nitrogen Application(s): Functional(Report Gene) Assay III. Description of Host Cell Line Organism: Homo sapiens, human Tissue: Peripheral blood Disease: Acute T cell leukemia Morphology: Lymphoblast Growth Properties: Suspension IV. Representative Data Figure 1.Detect Luciferase assay byUltra Luciferase Detection KitCBPH0001（we strongly suggest to purchase from Cobioer). Induction of NFkB activity by PMA with lonomycin in Jukart-NFkB Luciferase Reporter Cell. |
| Species | Homo sapiens, human |
| Full Name | NFkB-Luc/Jurkat Reporter Cell Line, NFkB-Luc/Jurkat |
| Parental Cell Line | Jurkat |
| Biosafety Level | BSL 2. Runtogen determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. |
| Expression Gene | Luciferase |
| Culture Method | RPMI-1640+10%FBS+800ug/ml hygromycin |
| Quality Control | NFkB-Luc/Jurkat Reporter Cell Line is negative for mycoplasma, bacteria, yeast, and fungi. |
| Shipping | Cryopreserved cells are shipped with dry ice |
| Storage | Upon arrival, Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Warranty | NFkB-Luc/Jurkat Reporter Cell Line is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product datasheet, website, and COA. |
| Disclaimers | This cell line is intended for laboratory research use only. It is not intended for any human or animal therapeutic use, any animal or human consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from Runtogen. |
Inquire the NFkB-Luc/Jurkat Reporter Cell Line by filling out the form below. We’ll get back to you within 24 hours with a quote.
Inquire the NFkB-Luc/Jurkat Reporter Cell Line by filling out the form below. We’ll get back to you within 24 hours with a quote.
