C2BBe1 Cell Line

Product Name	C2BBe1 Cell Line
Cat.No	RWT-902
Size	1*10^6 cells/vial
Description	The C2BBe1 (brush border expressing) cell line was cloned from the Caco-2 cell line by limiting dilution; the clone was selected on the basis of morphological homogeneity and exclusive apical villin localization; C2BBe1 cells form a polarized monolayer with an apical brush border (BB) morphologically comparable to that of the human colon; isolated BB contained the microvillar proteins villin, fimbrin, sucrase-isomaltase, BB myosin-1 and the terminal web proteins fodrin and myosin II ; the cells express substantial levels of BB myosin I similar to that of the human enterocyte; although clonal, and far more homogeneous than the parental Caco-2 cell line with respect to BB expression, these cells are still heterogenous for microvillar length, microvillar aggregation, and levels of expression of certain BB proteins.
Tissue	Colon
Species	Homo sapiens, human
Gender	Male, 72 years, Caucasian
Disease	Colorectal adenocarcinoma
Biosafety Level	BSL 1. Runtogen determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services.
Morphology	Epithelial-like Adherent
Culture Method	MEM+20%FBS+1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)
Quality Control	C2BBe1 Cell Line is negative for mycoplasma, bacteria, yeast, and fungi.
Shipping	Cryopreserved cells are shipped with dry ice
Storage	Upon arrival, Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
Warranty	C2BBe1 Cell Line is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product datasheet, website, and COA.
Disclaimers	Our cell line product is intended for laboratory research use only. It is not intended for any human or animal therapeutic use, any animal or human consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from Runtogen.

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